primary mouse bmscs primary mouse bone marrow mesenchymal stem cells bmscs  (Servicebio Inc)

Journal: BMC Musculoskeletal Disorders

Article Title: Bone marrow mesenchymal stem cell-derived exosomes promote osteoblast proliferation, migration and inhibit apoptosis by regulating KLF3-AS1/miR-338-3p

doi: 10.1186/s12891-024-07236-0

Figure Lengend Snippet: Identification of BMSCs-exosomes. (A) Size distribution of BMSCs-exosomes. (B) transmission electron microscopy image of BMSCs-exosomes. Scale bars: 100 nm. (C) Western blot results of exosome surface proteins

Article Snippet: Primary mouse bone marrow mesenchymal stem cells (BMSCs) were purchased from ATCC and cultured in a humidity-adjustable incubator containing 5% CO 2 at 37°C.

Techniques: Transmission Assay, Electron Microscopy, Western Blot

Journal: BMC Musculoskeletal Disorders

Article Title: Bone marrow mesenchymal stem cell-derived exosomes promote osteoblast proliferation, migration and inhibit apoptosis by regulating KLF3-AS1/miR-338-3p

doi: 10.1186/s12891-024-07236-0

Figure Lengend Snippet: Effects of BMSCs-exosomes on the function of MC3T3-E1 cells. (A) Screening of BMSCs-Exo concentration. (B) BMSCS-EXO-induced KLF3-AS1 elevation can be transfected with si-KLF3-AS1 inversion. BMSCs-Exo can promote the (C) cell viability and (D) migration of MC3T3-E1 cells and inhibit (E) cell apoptosis. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. control group; ## p < 0.01, ### p < 0.001 vs. BMSCs-Exo group ( n = 5, one-way ANOVA).

Article Snippet: Primary mouse bone marrow mesenchymal stem cells (BMSCs) were purchased from ATCC and cultured in a humidity-adjustable incubator containing 5% CO 2 at 37°C.

Techniques: Concentration Assay, Transfection, Migration, Control

Journal: BMC Musculoskeletal Disorders

Article Title: Bone marrow mesenchymal stem cell-derived exosomes promote osteoblast proliferation, migration and inhibit apoptosis by regulating KLF3-AS1/miR-338-3p

doi: 10.1186/s12891-024-07236-0

Figure Lengend Snippet: Effects of miR-338-3p in MC3T3-E1 cells incubated with BMSCs-Exo. (A) Transfection of miR-338-3p inhibitors down-regulated the expression level of miR-338-3p in BMSCs-Exo incubated MC3T3-E1 cells. The decrease of miR-338-3p can promote the (B) viability and (C) migration of BMSCs-Exo incubated MC3T3-E1 cells, and inhibit (D) cell apoptosis. *** p < 0.001 vs. Control group; ## p < 0.01, ### p < 0.001 vs. BMSC-Exos + si-NC group; & p < 0.05, && p < 0.01, &&& p < 0.001 vs. BMSC-Exos + si-KLF3-AS1 + inhibitor-NC group ( n = 5, one-way ANOVA).

Article Snippet: Primary mouse bone marrow mesenchymal stem cells (BMSCs) were purchased from ATCC and cultured in a humidity-adjustable incubator containing 5% CO 2 at 37°C.

Techniques: Incubation, Transfection, Expressing, Migration, Control

Journal: BMC Musculoskeletal Disorders

Article Title: Bone marrow mesenchymal stem cell-derived exosomes promote osteoblast proliferation, migration and inhibit apoptosis by regulating KLF3-AS1/miR-338-3p

doi: 10.1186/s12891-024-07236-0

Figure Lengend Snippet: PTEN is a target gene of miR-338-3p. (A) Complementary sites of miR-338-3p and PTEN. (B) luciferase reporter gene assay. *** p < 0.001 vs. Control group ( n = 5, one-way ANOVA). The expression level of PTEN in serum was down-regulated in patients with (C) hand fracture and (D) intraarticular fracture (Healthy individuals = 65, Hand fracture = 35, Intra-articular fracture = 30). *** p < 0.001 vs. Healthy individuals (Independent sample t test). (E) The content of PTEN was increased in BMSCs-Exo incubated cells. *** p < 0.001 vs. control group; ### p < 0.001 vs. BMSCs-Exo + si-NC group; &&& p < 0.001 vs. BMSCs-Exo + si-KLF3-AS1 + inhibitor-NC group ( n = 5, one-way ANOVA).

Article Snippet: Primary mouse bone marrow mesenchymal stem cells (BMSCs) were purchased from ATCC and cultured in a humidity-adjustable incubator containing 5% CO 2 at 37°C.

Techniques: Luciferase, Reporter Gene Assay, Control, Expressing, Incubation

Journal: Journal of Nanobiotechnology

Article Title: Supramolecular delivery of senolytics enables targeted anti-senescence therapy and accelerated fracture healing

doi: 10.1186/s12951-026-04138-2

Figure Lengend Snippet: Asp 10 SAC4A enables hypoxia-activated senolysis and promotes osteogenic differentiation in vitro. A ) Heatmap of combination index (CI) values at different D: Q ratios and fraction affected (Fa) levels, showing synergistic effects of Dasatinib and Quercetin (DQ). B ) Cell viability curves of normal BMSCs, senescent BMSCs (Sn-BMSCs), and Sn-BMSCs under hypoxia treated with vehicle, free DQ, Asp 10 SAC4A, or DQ@Asp 10 SAC4A. C ) Representative SA-β-Gal staining images of senescent cells treated with different formulations under normoxic and hypoxic conditions, and quantification of SA-β-Gal-positive area (%) ( n = 4/group). Scale bar: 100 μm. D–F ) Representative Western blot images ( D ) and quantitative analyses ( E , F ) of senescence markers P16 and P21 expression in different treatment groups ( n = 3/group). G ) Representative alkaline phosphatase (ALP, upper panel) and Alizarin Red staining (ARS, lower panel) images demonstrating osteogenic differentiation after indicated treatments under normoxic and hypoxic conditions. H–J ) Representative Western blot images ( H ) and quantitative analyses ( I , J ) showing protein expression levels of osteogenic markers RUNX2 and osteopontin (OPN) ( n = 3/group). (Data are presented as mean ± SD; * P < 0.05, ** P < 0.01, *** P < 0.001; n = 3–4/group)

Article Snippet: Primary mouse bone marrow mesenchymal stem cells (BMSCs) were purchased from Servicebio (Catalog number STCC6011P).

Techniques: In Vitro, Staining, Western Blot, Expressing